I would never tile straight onto any timber in any form. Whether there is any bounce or not wood will move with changes in temperature. I would always recommend either Hardi backer or at the very least a construction board. Both stuck with flexible adhesive and screwed every 300mm. The tape needs to be alkaline resistant to avoid breaking down over time. In effect the floor needs to be tanked with the hardibacker to avoid water finding its way through to the timber substrate.